GenCefe's recombinant protein platform integrates gene synthesis, codon optimization, protein expression, and purification into a streamlined gene-to-protein workflow. By combining expertise in molecular biology and protein production, we support efficient development of recombinant proteins and antibodies for a wide range of research applications.

Multiple expression systems are available, including bacterial, yeast, insect, mammalian, and cell-free platforms, allowing expression strategies to be tailored to different protein characteristics and project requirements. For challenging targets such as membrane proteins, toxic proteins, and difficult-to-express proteins, our proprietary cell-free expression platform provides an additional solution beyond conventional expression systems.

Platform Advantages

· Integrated gene-to-protein workflow from gene synthesis to purified protein

· Multiple expression platforms for diverse protein types and application needs

· Proprietary cell-free technology for challenging protein targets

· Recombinant protein and antibody expression capabilities

· Sequence optimization and construct design support

· Standardized workflows operating under ISO 9001 and ISO 13485 quality systems

GenCefe is committed to ensuring both product quality and data confidentiality throughout recombinant protein projects. Our gene-to-protein workflows, from gene design and synthesis to protein expression and purification, operate under ISO 9001 and ISO 13485 certified quality management systems to ensure traceability, process control, and consistent project execution. Strict confidentiality policies are applied to protect all customer-provided information and project data.

Quality Control

· Standardized process control across gene synthesis, protein expression, and purification workflows

· Protein purity and integrity assessment by SDS-PAGE analysis

· Protein concentration determination using validated analytical methods

· Optional functional assays, such as activity or binding analysis, depending on project requirements

· Additional quality testing available based on protein type and application needs

· Comprehensive QC documentation provided to support traceability and reproducibility

Confidentiality

· Secure handling of all customer-provided sequences and project information

· Restricted access to project data limited to authorized personnel only

· No disclosure of project information to third parties without customer consent

· NDA agreements available upon request

GenCefe's recombinant protein expression and purification services are built around an integrated gene-to-protein workflow, designed to support structural studies, functional assays, and diagnostic development. By combining gene synthesis, codon optimization, protein expression, and purification, we provide a streamlined process from sequence to functional protein with improved consistency and efficiency.

Our platform also includes a proprietary cell-free expression system for challenging targets such as membrane proteins, toxic proteins, and difficult-to-express proteins. In addition, multiple expression systems—including bacterial, yeast, insect, mammalian, and cell-free platforms—enable flexible strategy design based on protein characteristics and project requirements. Recombinant protein and antibody expression are both supported within the same technology framework.

Value Highlights:

· Integrated gene-to-protein workflow from gene design to protein purification

· Proprietary cell-free platform for difficult-to-express and toxic proteins

· Recombinant protein and antibody expression capability

· Multiple expression systems for flexible strategy selection

· Batch-to-batch consistency supported by standardized workflows and QC documentation

· Technical support throughout the project to ensure experimental success

1. 7.4-Fold increase in eukaryotic protein expression in E. coli

The three clones containing optimized SKN-1 gene showed a 6-fold, 7.2-fold, and 9-fold increase separately, with an average of 7.4-fold increase, over the clones containing wild-type.

Figure1. Left: Protein electrophoresis of bacteria lysate expressing wild-type and optimized SKN-1 gene. Right: protein quantitation by Western Blot.

2. Functional Membrane Protein Expression-Cell-Free Expression System

Figure 2. Prof. Kai, co-founder of GenCefe, published a research result using an innovative cell-free expression system, successfully reconstituting the prenylated polarity hub Cdc42 and its regulatory protein in vitro, implementing a key membrane switch.

Ref: Kai L, Sonal, Heermann T, et al. Reconstitution of a reversible membrane switch via prenylation by one-pot cell-free expression[J]. ACS Synthetic Biology, 2022, 12(1): 108-119. https://doi.org/10.1021/acssynbio.2c00406

GenCefe streamlines recombinant protein ordering from sequence submission to final delivery, enabling a clear and transparent project workflow. Submit your request via email or online form—our scientific team will provide free assessment and project design recommendations within one business day. 

1. Request a Quote: Email [email protected] with your target sequence and project requirements, including full-length or domain design, and tag or tag-free preferences.

2. Gene Design & Codon Optimization: Sequence analysis, construct design, and codon optimization are performed to support optimal expression in the selected system, followed by gene synthesis for downstream protein production.

3. Small-Scale Expression Screening: Initial small-scale expression is conducted to evaluate protein expression level, solubility, and construct performance, helping to de-risk downstream scale-up and select optimal expression conditions.

4. Scale-Up Expression & Purification: Optimized constructs are used for scaled protein production in bacterial, yeast, insect, mammalian, or cell-free systems, followed by standardized purification workflows under controlled conditions.

5. Quality Control & Documentation: Purified proteins are analyzed using SDS-PAGE and concentration measurement, with optional functional or binding assays depending on project requirements. Comprehensive QC documentation is provided under ISO 9001 and ISO 13485 certified systems.

6. Global Delivery: Purified recombinant proteins are delivered with complete batch traceability and secure international shipping.

Online Quote: [Submit Sequence Now]

Contact by email: [email protected]

GenCefe's service architecture is designed to address key technical challenges in recombinant protein development, including sequence complexity, expression variability, protein aggregation, and purification consistency. Our scientific team combines expertise in molecular biology and biochemistry with standardized workflows to ensure each project progresses with clarity, efficiency, and reproducibility.

By integrating gene design, protein expression, and purification within a unified workflow, we reduce process fragmentation and support consistent performance across projects. All processes operate under ISO 9001 and ISO 13485 certified quality management systems, ensuring traceability, controlled documentation, and reliable support for research and diagnostic applications.

With strong technical expertise and standardized operational systems, GenCefe provides a reliable platform for recombinant protein development projects.

GenCefe's recombinant protein expression and purification services support a wide range of research and development needs. Delivered proteins are used in structural studies, functional research, assay development, and early-stage therapeutic research.

Structural Biology & Protein Characterization

· Proteins used in X-ray crystallography, cryo-EM, and structural analysis

· Supporting studies of protein folding, stability, and molecular interactions

Functional & Mechanistic Studies

· Enzymes and regulatory proteins for activity assays and signaling pathway research

· Applied in biochemical and cell-based experimental systems

Assay Development

· Recombinant antigens and reference proteins for ELISA, immunoassays, and diagnostic development

· Used in assay optimization and performance evaluation

Antibody Research & Target Validation

· Recombinant proteins used for antibody discovery, screening, and target validation

· Supporting early-stage therapeutic and translational research

Specialized Protein Expression

· Membrane proteins, toxic proteins, and proteins with non-standard amino acids

· Produced using cell-free expression systems when conventional expression is not suitable

We offer bacterial (E. coli), yeast, insect cell, mammalian cell (CHO and HEK293), and a proprietary cell-free expression system. System selection is based on protein characteristics and project requirements.

System selection depends on factors such as protein size, complexity, solubility, post-translational modification requirements, and expression goals. Our scientific team can provide evaluation and recommendations during project design.

Expression System Selection Guide:

Yes, we support both recombinant protein and antibody expression for research and early-stage development applications.

Yes. These challenging targets can be addressed using our proprietary cell-free expression platform or other optimized expression strategies based on project requirements.

Projects generally include gene design, codon optimization, gene synthesis, small-scale expression screening, scale-up production, purification, quality control, and final delivery.

We support flexible production scales ranging from milligram quantities for research use to gram-level production depending on project requirements.

Purity levels are project-dependent. Depending on feasibility and application needs, proteins can typically be delivered at >90% purity, with higher purity levels such as >95% or >98% achievable in selected cases.