One of the most exciting advances in peptide engineering is the development of stapled peptides—modified peptides that are chemically "stapled" to preserve their alpha-helical structure, thereby enhancing their biological activity and therapeutic potential.

At GenCefe Biotech, we offer custom stapled peptide synthesis, helping researchers design peptides that are more stable, bioavailable, and functionally active in complex biological systems.

What Are Stapled Peptides?
Stapled peptides are linear peptides that have been chemically crosslinked between two residues (commonly at i and i+4 or i+7 positions) using hydrocarbon or other linkers. This structural reinforcement enhances alpha-helical stability, protease resistance, and cellular uptake.

Key Benefits of Stapled Peptides:

1. Improved Structural Stability
Stapling preserves the bioactive conformation, especially for peptides that mimic protein–protein interaction (PPI) domains.

2. Increased Resistance to Degradation
Crosslinking protects against proteolytic enzymes, increasing the half-life of the peptide in biological fluids.

3. Enhanced Membrane Permeability
Stapled peptides often show better intracellular delivery, making them suitable for targeting intracellular proteins.

4. Better Therapeutic Efficacy
By maintaining optimal binding conformation, stapled peptides achieve higher affinity and prolonged activity in vivo.

Applications of Stapled Peptides:

●  Inhibition of protein–protein interactions in cancer and inflammation

●  Intracellular signaling modulation

●  Viral entry inhibition

●  Next-generation peptide drugs for diseases previously considered “undruggable”

GenCefe’s Stapled Peptide Services:

●  Custom design and synthesis of stapled peptides with various crosslinking strategies

●  Alpha-helical structure optimization based on target interaction sites

●  Incorporation of non-natural amino acids as needed

●  Analytical support including CD, MS, and HPLC characterization

Stapled peptides represent the cutting edge of peptide therapeutics, and GenCefe Biotech is proud to help researchers bring these promising molecules from design to application.

Need Custom Peptides? Contact Us Today

For inquiries or quotations, reach out to our peptide experts at [email protected]. GenCefe Biotech—your trusted partner in peptide synthesis and modification.