qPCR (quantitative polymerase chain reaction), also called real-time PCR, is a molecular biology technique used to quickly and sensitively measure the amount of a specific gene in a DNA or RNA sample. Primers and probes are one of the key factors affecting the success of qPCR and the reliability of its results.

GenCefe Biotech has established an advanced oligo synthesis and modification technology platform with an experienced technical team to provide customized qPCR primers and probes. We are committed to assisting your research and diagnostic applications with high accuracy and reliability of qPCR primers and probes.

Service Advantages:

• ISO9001 certified, GMP-like clean room, strict control of pollution sources, avoiding NTC interference

• Advanced production technology ensures product accuracy, high purity, and stable fluorescent labeling

• Standardized operation and strict quality control to ensure batch-to-batch stability of primers/probes

qPCR Primers & Probes Case Studies:

Fluorescence signal analysis compared to competitors

Cy5-labeled probes synthesized by GenCefe and Company B were added to the two amplification systems for qPCR verification. The reaction systems were completely consistent except for the probes.

	GenCefe	Company B
Fluorescence signal	Higher	Lower
Fluorescence background signal	Lower	Higher
Ct value	Basically the same

Probes designed and synthesized by GenCefe show excellent linear range and ideal repeatability

qPCR Primers & Probes Service Specifications:

Please download and fill out the oligo order form and send it via email to  [email protected] . Please note the minimum order size of custom oligo synthesis is $50/order.

Modification/Labeling/Dual-Labeling: view the full list