Gene Synthesis
Custom gene synthesis solutions with optimized design and reliable quality.
5+ Years
99%+
300,000+
20+ Countries
GenCefe Biotech provides custom gene synthesis for natural and codon-optimized sequences, gene libraries, complex sequences, long sequences, and other customized designs. Synthesized genes can be cloned into specified vectors and sites according to project requirements, with flexible options to support different research and application needs.
From sequence submission and technical evaluation to gene synthesis, cloning, and quality control, GenCefe provides integrated project support with a successful delivery rate of up to >99%. Turnaround times start from 5 business days, with QC documentation provided according to service specifications.
Key Features
Fast Turnaround--Synthesis turnaround starts from 5 business days.
Complex Sequence Synthesis--Support for challenging sequences, including GC-rich, repetitive, and ultra-long genes.
Technical Support--Professional technical support throughout the synthesis process, from sequence evaluation to project delivery.
Sequence Quality Control-- Gene synthesis workflows are operated under ISO 9001 and ISO 13485 certified quality management systems.
Gene Synthesis Service Specifications
| Gene Length | Turnaround Time (Business Day) | Price* | Order |
| <=526 bp | 5-8 | $79/gene | Get a Quote |
| 527~1500 bp | 6-10 | $0.15/bp | |
| 1501~3000 bp | 10-15 | $0.15/bp | |
| 3001~5000 bp | 15-20 | $0.23/bp | |
| 5001~7000 bp | 20-25 | Get a Quote | |
| >7000 bp | Get a Quote | Get a Quote |
* The minimum charge for gene synthesis services is $79/order.
Delivery Specifications
4 μg of lyophilized plasmid
Sequence chromatograms covering your gene (e-version)
Construct map of the plasmid (e-version)
Quality assurance certificate
Service Process
1. Optimization & Project Design: Our team evaluates the sequence and develops an appropriate gene synthesis strategy, including sequence optimization when required.
2. Oligo Synthesis: Oligonucleotides are synthesized according to the designed sequence and project specifications.
3. Gene Assembly: Synthesized oligonucleotides are assembled to generate the target gene sequence.
4. Cloning: The assembled gene is cloned into the specified vector according to the project requirements.
5. Quality Control: Sequence verification and applicable quality checks are performed to confirm the quality and accuracy of the final construct.
6. Delivery: Final products are prepared and shipped with order tracking and professional logistics support.
GenCefe's gene synthesis workflow integrates sequence optimization, oligo synthesis, gene assembly, cloning, and quality control to support accurate and reliable gene construction.
Case Study
Difficult-to-synthesis gene sequence (PolyA-rich)
The gene sequence containing 110 consecutive PolyA has been successfully synthesized and verified by sequencing. And the 100% sequence correct plasmids were delivered to customers.
Difficulties of the Project
The sequence contains a large repeat structure, including a continuous PolyA structure of up to 110 bases. Such consecutive single-base repeats can lead to plasmid instability and abnormal sequencing signals.
GenCefe Solutions
A specific synthetic scheme was designed for this sequence.
Optimized construction and cloning protocols to reduce mutations.
Optimized sequencing protocols to ensure accurate validation.
“We availed gene synthesis services form Gencefe for constructs of our propriety mammalian expression vectors and it matched 100% sequence we provided. Successfully we expressed several proteins and antibodies using the vectors we received from Gencefe.
In another instance, we were trying to clones chimeric protein gene and were facing challenges due to multiple G4S linkers, here again we availed the services of Gencefe, they have codon optimized the sequence, synthesized and cloned in pET21 a vector, we expressed the protein and expression was pretty high.
Based on the experience, I recommended few of friends in Industry and they also expressed satisfaction with their services, I hope the Gencefe maintain same quality and good services in future too”
Gene Synthesis Services
Explore GenCefe’s gene synthesis capabilities, including custom synthesis options, sequence optimization, quality control, and service workflows.
