STR Primers
Short Tandem Repeat (STR) is a DNA repeat sequence composed of 2-6 base units in the genome, also called microsatellite DNA or simple repeat sequence (SSR). Since the number of core unit repeats is variable and abundant among individuals, STR is highly polymorphic and widely exists in the coding and non-coding regions of eukaryotic genomes. As an important genetic marker system, STR It is widely used in many fields such as gene mapping, tumor analysis, forensic identification, paternity testing and genetic analysis.
Based on our advanced oligonucleotide synthesis and modification technology platform, GenCefe's professional team has optimized the dye and purification process, and developed STR primer quality standards that meet forensic identification applications, providing you with high-quality fluorescently labeled STR primers.
Case Study:
Difficulties of the Project:
Multiplex amplification may suffer from channel bleeding, resulting in non-specific amplification
Fluorescent dyes have poor stability
Conventional purification cannot meet purity requirements, and fluorescence residue cannot be avoided.
GenCefe Solutions:
Optimize dyes and their spatial structures to improve fluorescence signal stability;
Customized purification process to improve purity and avoid fluorescence residue
Sanger sequencing verification to ensure no fluorescence residue or loss
STR Primers Service Specifications:
| Modifications | Purification Method | Length | Deliverables |
| 6-FAM HEX TAMRA ROX Others | HPLC | 11-59 nt | Tube or 96-well plate Dry powder COA |
Please download and fill out the oligo order form and send it via email to [email protected]. Please note the minimum order size of custom oligo synthesis is $50/order.
