Plasmid Preparation
Flexible plasmid preparation tailored to your specific needs.
GenCefe provides flexible plasmid preparation services at scales ranging from micrograms to milligrams and grams, with strict quality control to support consistent batch-to-batch performance. Endotoxin removal is available according to project requirements, supporting plasmid preparation for research, transfection, and other applications.
Key Features
Flexible Production Scale: Microgram- to gram-scale plasmid preparation.
Strict Quality Control: Multiple QC standards and customized QC items available upon request.
Fast Turnaround: Plasmid preparation completed in as few as 3 business days.
Comprehensive Services: Integrated support from gene synthesis and custom cloning to plasmid preparation.
Plasmid Preparation Service Specifications
| Research Level | Transfection Level | |
| Endotoxin Level | NA | <0.05EU/μg or <0.005EU/μg |
| Scale | μg to g level | |
| Turnaround Time | Starting from 3 business days | |
| QC Method | Standard QC Items Research-level QC standards | Standard QC Items Transfection-level QC standards |
| Recommended Applications | Molecular biology research: gene cloning, mutagenesis, DNA library construction, transient protein expression, etc. | Industrial applications: mammalian cell transfection, viral packaging, protein expression, antibody production, GCT, vaccine development, etc. |
| Deliverables | Plasmid DNA, QC Report | |
| Price | Get a Quote | |
Plasmid Preparation QC Standards
QC Items | Detection Method | Standards | ||
Research Level | Transfection Level | |||
Standard Items | Appearance | Visual inspection | Transparent without impurities | Transparent without impurities |
A260/280 | Micro-spectrophotometer | 1.8-2.0 | 1.8-2.0 | |
Concentration | 1ug/uL | 1ug/uL | ||
Supercoil ratio analysis | Agarose electrophoresis grayscale analysis | >50% | >90% | |
Genomic DNA | < 15% of total plasmid DNA | < 15% of total plasmid DNA | ||
RNA residue | Agarose electrophoresis analysis | Undetectable | Undetectable | |
Restriction enzyme validation | Agarose gel electrophoresis analysis of digested products | No stray bands | No stray bands | |
Sequencing verification | Sanger sequencing | 100% correct | 100% correct | |
Endotoxin detection | Endotoxin analysis kit | NA | ≤50EU/mg | |
Custom Items | Bioburden | Plate coating after filtration | No colonies after 48 hours | No colonies after 48 hours |
Others | Customizable according to customer's needs | |||
Service Process
1. Starting Plasmid: Plasmids synthesized by GenCefe or provided by customers are assessed for suitability before entering the preparation process.
2. Amplification: The plasmid is amplified in an appropriate host system to obtain the required amount of plasmid DNA.
3. Bacterial Lysis: Bacterial cells are harvested and lysed to release the plasmid DNA.
4. DNA Purification: Plasmid DNA is purified using standardized procedures to achieve the required quantity and quality.
5. Quality Control: Quality checks are performed according to project requirements, with customized QC items available upon request.
6. Delivery: Final plasmid DNA is prepared and shipped with order tracking and logistics support.
GenCefe's plasmid preparation workflow integrates plasmid amplification, bacterial lysis, DNA purification, and quality control to provide plasmid DNA tailored to your specific requirements.
Gene Synthesis Services
Explore GenCefe’s gene synthesis capabilities, including custom synthesis options, sequence optimization, quality control, and service workflows.
