A typical CRISPR-Cas9 experiment requires two components, so it is important to select the appropriate way to construct CRISPR sgRNA and Cas9 vector for your downstream applications.

There are two common ways to construct CRISPR-Cas9 plasmid, the first option is to clone sgRNA and Cas9 into one plasmid. Also, sgRNA and Cas9 can be cloned into two individual plasmids. And then, deliver the plasmid to transfect the cells through electroporation/lentiviral transfection to edit target genes precisely. GenCefe offers an all-in-one plasmid to express sgRNA and Cas9 together. Alternatively, we can also construct two separate plasmids for sgRNA and Cas9, respectively.

The Advantages of two CRISPR-Cas9 plasmids

All-in-one CRISPR-Cas9 system	Two-plasmids CRISPR-Cas9 syste
Both sgRNA and Cas9 are expressed simultaneously Easiest to implement during the experiment	Flexibility to adjust the ratio of sgRNA and Cas9

Deliverable and Quality Control Documents

• 4 μg lyophilized plasmid DNA
• Sequence chromatograms
• Plasmid construct map
• QC data