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GenCefe provides you with high-quality plasmid preparation services, flexible
production scale from micrograms, milligrams to grams; strict quality control
to ensure batch-to-batch stability. We can perform endotoxin removal according
to customer requirements to meet different plasmid production needs of
research level and transfection level.
Plasmid Preparation Service Specifications
| Research Level | Transfection Level | |
| Endotoxin Level | NA | <0.05EU/μg or <0.005EU/μg |
| Scale | μg to g level | |
| Turnaround Time | Starting from 3 business days | |
| QC Method |
Standard QC Items
Research-level QC standards |
Standard QC Items
Transfection-level QC standards |
| Recommended Applications | Molecular biology research: gene cloning, mutagenesis, DNA library construction, transient protein expression, etc. | Industrial applications: mammalian cell transfection, viral packaging, protein expression, antibody production, GCT, vaccine development, etc. |
| Deliverables | Plasmid DNA, QC Report | |
| Price | Get a Quote | |
Key Features
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Flexible production scale: microgram to gram level plasmid preparation
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Strict quality control methods: multiple QC standards and customized QC items are available upon request
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Fast turnaround time: deliver the plasmid DNA as soon as 3 business days
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Comprehensive services: from gene synthesis to custom cloning and plasmid preparation
Service Process
Plasmid Preparation QC Standards
|
QC Items |
Detection Method |
Standards |
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|
Research Level |
Transfection Level |
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|
Standard Items |
Appearance |
Visual inspection |
Transparent without impurities |
Transparent without impurities |
|
A260/280 |
Micro-spectrophotometer |
1.8-2.0 |
1.8-2.0 |
|
|
Concentration |
1ug/uL |
1ug/uL |
||
|
Supercoil ratio analysis |
Agarose electrophoresis grayscale analysis |
>50% |
>90% |
|
|
Genomic DNA |
< 15% of total plasmid DNA |
< 15% of total plasmid DNA |
||
|
RNA residue |
Agarose electrophoresis analysis |
Undetectable |
Undetectable |
|
|
Restriction Enzyme Validation |
Agarose gel electrophoresis analysis of digested products |
No stray bands |
No stray bands |
|
|
Sequencing verification |
Sanger sequencing |
100% correct |
100% correct |
|
|
Endotoxin Residue Analysis |
Endotoxin Analysis Kit |
NA |
≤50EU/mg |
|
|
Custom Items |
Bioburden |
Plate coating after filtration |
No colonies after 48 hours |
No colonies after 48 hours |
|
Others |
Customizable according to customer's needs |
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| REQUEST A QUOTE | ||
|---|---|---|
| PHONE | ONLINE FORM | |
| [email protected] | +1 408-828-0438 | ONLINE QUOTE SUBMISSION |
