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GenCefe provides customized DNA oligo modification/labeling services, including more than 100 modifications such as biotin, digoxigenin, phosphate, thiol, sulfhydryl, amino, special bases, and a variety of fluorescent groups. 100% of the delivered modified probes/primers have passed mass spectrometry detection to ensure accuracy and purity, meeting the needs of scientific research and industrial-grade modified primers/probes.
Oligo Modification Position:
DNA Oligo Modification Case Study:
125 nt, Spacer18 modification, multiple Poly-T sequences, purity>95.58%,
molecular weight bias<0.05%
DNA Modification Groups:
| Rare Base | Fluorescence | Fluorescence | Intermediate | Others |
|
dI
dU m5C m6A 2' -O-Methyl base RNA base LNA |
5' Cy3
3' Cy3 5' Cy5 3' Cy5 5' FAM 3' FAM 5' HEX 5' TET 5'-FITC 3'-FITC 5' 6-JOE 3' 6-JOE 5' ROX 3' ROX 5' TAMRA 3' TAMRA 3' -HEX 3' -TET 5' Helix Flour555 3' Helix Flour555 5' Helix Flour575 3' Helix Flour575 5' Texas Red 3' Texas Red 5' Quasar 570 3' Quasar 570 5' Quasar 670 3' Quasar 670 |
5' Cy3.5
3' Cy3.5 5' Cy5.5NS 3' Cy5.5NS 5' Cy7NS 3' Cy7NS 5'&3' CAL Fluor Red 590 5'&3' CAL Fluor Red 610 5'&3' DABCYL 5'&3' AMCA 5'&3' DEAC 5'&3' MCA 5'-VIC 5'-SIMA |
Int NH2 dT
Int Biotin dT Int Cy3 dT Int Cy5 dT Int TAMRA dT Int ROX dT Int FAM dT Int HEX dT Int Dig dT Int CHCH dT Int DBCO dT Int Azide dT Int BHQ1 dT Int BHQ2 dT Int HS-SH C6 Int Alexa Flour dT Int Azobenzene Int Ferrocene dT dT- LC RED-610 dT- LC RED-640 dT- LC RED-705 dC-Fluorescein dC-Tamra dC-Amino dC-Rox dC-Digoxin dC-FITC dC-Texas Red |
Azide
DBCO Spacer9 Spacer(C12) Spacer C3 Spacer C18 dSpacer Inverted Base 5' -Carboxy C10 5' -Acridine 3' ddC Alexa Flour 647 Yakima Yellow Epoch Pyrene |
| Phosphorothioate | ||||
| S | ||||
| Phosphorylation | ||||
|
5' PHO
3' PHO |
||||
| Amino Linkers | ||||
|
5' NH2 C6
3' NH2 C7 5' NH2 C12 |
||||
| Thiol | Quenchers | |||
|
3' SH C3
5' SH C6 |
3' DABCYL
3' TAMRA 3' BHQ 1 3' BHQ 2 3' BHQ3 3' -MGB 3' eclipse |
|||
| Biotin | ||||
|
5' Biotin
5' Biotin TEG 3' Biotin 3' Biotin TEG |
||||
| Digoxigenin | ||||
|
5' DIG
3' DIG |
Dual-Labeling Probes:
|
TAMRA
|
ECLIPS | BHQ1 | BHQ3 |
|
5' FAM-3' TAMRA
5' HEX-3' TAMRA 5' TET-3' TAMRA 5' JOE-3' TAMRA 5' VIC-3' TAMRA |
5' FAM-3' ECLIPS
5' HEX-3' ECLIPS 5' TAMRA-3' Eclipse 5' ROX-3' Eclipse 5' TET-3' Eclipse 5' JOE-3' Eclipse |
5' FAM-3' BHQ1
5' VIC-3' BHQ1 5' HEX-3' BHQ1 5' JOE-3' BHQ1 5' TET-3' BHQ-1 |
5' Quasar 670-3' BHQ3
5' CY5-3' BHQ3 |
| MGB | Dabcyl | BHQ2 | Others |
|
5' FAM-3' MGB
5' HEX-3' MGB 5' TET-3' MGB 5' JOE-3' MGB 5' ROX-3' MGB 5' Texas Red-3' MGB 5' Quasar 670-3' MGB 5' VIC-3' MGB |
5' FAM-3' DABCYL
5' HEX-3' DABCYL 5' TET-3' DABCYL 5' JOE-3' DABCYL 5' TAMRA-3' DABCYL 5' Texas Red-3' Dabcyl 5' ROX-3' Dabcyl 5' CY3-3' DABCYL 5' Cy5-3' DABCYL |
5' Cy5-3' BHQ2
5' Cy3-3' BHQ2 5' ROX-3' BHQ2 5' TET-3' BHQ2 5' JOE-3' BHQ2 5' HEX-3' BHQ2 5' TAMRA-3' BHQ2 5' Texas Red-3' BHQ2 5' Quasar 670-3' BHQ2 5' -FAM and 3' -BHQ2 |
5' Phos and 3' FAM
5' TAMRA and 3' Phos 5' C6-Biotin and 3' Biotin 5' C6-Biotin and 3' Cy5 5' C6-NH2 and 3' C3-Fam 5' C6-NH2 and 3' Cy3 5'Cy5 and SS 5' Cy5 and 3' C7-NH2 5' Cy5 and 3' SH 5' Dabcyl and 3' C3-Fam 5' DlG and 3' Digoxin 5' SH and 3' Cy3 5' Fam and 3' C3-Fam 5' TAMRA and 3' C3-Fam |
Please download and fill out the oligo order form and send it via email to [email protected] . Please note the minimum order size of custom oligo synthesis is $50/order.
| REQUEST A QUOTE | ||
|---|---|---|
| PHONE | ONLINE FORM | |
| [email protected] | +1 408-828-0438 | ONLINE QUOTE SUBMISSION |
