Oligonucleotide Synthesis and Modifications
GenCefe Biotech has established a complete oligonucleotide synthesis and modification platform, with advanced synthesizers of different fluxes to meet the synthesis requirements of different specifications. Professional R&D and technical teams and mature synthesis, purification, and modification methods, to provide high-quality oligonucleotides for nucleic acid drug researchers. We provide siRNA, antisense oligonucleotide, miRNA, nucleic acid aptamer, and sgRNA synthesis, and can also customize the synthesis of various nucleic acid sequences. According to the needs of customers, we can design and synthesize RNA oligos of different lengths, different forms, and with various modifications, and we can also synthesize RNA sequences designed by customers. The synthesis specifications are flexible, and OD, mg, gram, and hectogram levels can be selected for synthesis. All products are purified by HPLC, implement strict QC standards, and meet ISO9001 quality management system certification standards.
Oligonucleotide Synthesis
| Type | Product Name | Size | Purification | Turnaround Time (days) |
| siRNA | siRNA | OD to hectogram | HPLC | Starting from 4 days |
| Modified siRNA | Get a Quote | Get a Quote | ||
| siRNA controls | 1 OD | In stock | ||
siRNA Guaranteed Package
| HPLC | Starting from 5 days | ||
| miRNA (in vitro) | microRNA mimic | Starting from 2 OD | HPLC | Starting from 4 days |
| microRNA inhibitor | ||||
| microRNA mimics N.C. | ||||
| microRNA inhibitor N.C. | ||||
| miRNA (in vivo) | microRNA agomir | Starting from 2 OD | HPLC | Starting from 7 days |
| microRNA antagomir | ||||
| microRNA agomir N.C. | ||||
| microRNA antagomir N.C. | ||||
Antisense Oligonucleotides (ASOs) | ASOs | μg to gram | HPLC | Get a Quote |
| Modified ASOs | ||||
| Nucleic acid aptamer | Aptamer | μg to gram | HPLC | Get a Quote |
| Modified Aptamer | ||||
| sgRNA | Chemically synthesized sgRNA | Starting from 2 OD | HPLC | Starting from 7 days |
Chemical Modification and Fluorescent Labeling Services
| Nucleic Acid Type | Chemical Modification and Fluorescent Labeling Services |
| siRNA | Sugar Modification: 2'-Ome, 2'-F, etc. Backbone Modification: PS (phosphorothioate) modification, etc. End modification: Chol (cholesterol), Biotin, Thiol, NH2, etc. Fluorescent labeling: Cy3, Cy5, etc. Customized chemical modification and fluorescent labeling proposal design |
| Antisense (ASO) | Sugar Modification: 2'-OMe, 2'-MOE, 2'-F, LNA, etc. Base Modification: methylated cytosine (5-Me-dC), etc. Backbone Modification: PS (phosphorothioate) modification, etc. End modification: Chol (cholesterol), etc. |
| Aptamer | Sugar Modification: 2'-OMe, 2'-NH2, 2'-F, LNA, etc. Base Modification: methylated cytosine (5-Me-dC), etc. Backbone Modification: PS (phosphorothioate) modification, etc. End modification: 3'-inverted thymidine, Chol (cholesterol), Biotin, Thiol, NH2, fluorescent dyes, etc. |
| Others | Custom chemical modification and fluorescent labeling services |
Delivery System Customization and Conjugation Services
| Delivery System | Features | Services |
| GalNAc (N-acetylgalactosamine) | GalNAc is the targeting ligand of the sialic acid receptor (ASGPR), which is mainly expressed on the surface of hepatic parenchymal cells. The conjugate of nucleic acid and GalNAc enters the cell through endocytosis to function. | Custom GalNAc Synthesis GalNAc-siRNA Synthesis GalNAc-ASO Synthesis GalNAc-miRNA Synthesis Customized design and synthesis of GalNAc delivery proposal |
| Peptide | Peptides have better membrane permeability and strong resistance to degradation. Therefore, conjugating oligonucleotides with various biological functions to peptides can not only improve the biological activity of oligonucleotides but also enhance their cell permeability. | Peptide Synthesis (drug peptides, custom peptides, RGD cyclic peptides, etc.) Peptide-siRNA Conjugation Peptide-ASO Conjugation Peptide-DNA Conjugation RGD-siRNA Conjugation |
| Lipid | LNP not only prevents degradation of delivered oligonucleotides and prolongs circulation time, but also facilitates oligonucleotide uptake, enhances intracellular transport, and facilitates endosomal escape. | LNP-siRNA Conjugation LNP-ASO Conjugation |
| PEG (polyethylene glycol) | The half-life of PEG-modified ASO in the body is significantly prolonged, and PEG can reduce the excretion rate of oligonucleotides and promote the uptake of nucleic acid drugs by corresponding cells. | PEG-Aptamer Conjugation PEG-siRNA Conjugation PEG-ASO Conjugation |
| Others | / | Custom delivery proposal design and synthesis |
QC Standards and Custom QC Services
GenCefe Biotech is committed to providing high-quality oligonucleotide products and services; we perform HPLC detection and provide COA files for all of our oligonucleotide products by default. There’s also a variety of customized QC service items to meet the specific needs of users.
Customized QC items (additional fee will be applied)
Molecular weight determination of oligonucleotides
Sequence confirmation of oligonucleotides
Impurity identification of oligonucleotides
Melting temperature (Tm) of oligonucleotide
IEX-HPLC Purity of Oligonucleotides
RP-HPLC Purity of Oligonucleotides
SEC-HPLC Purity of Oligonucleotides
Endotoxin content of oligonucleotide
