RNA Synthesis-RNA Modifications

RNA Modifications

The delivery method and efficiency are the key factors for whether small nucleic acid drugs can enter cells and play a role. There are several delivery approaches, the naked RNA modification delivery technology, liposome nano-delivery technology, conjugated delivery system (small molecule ligands, antibodies, and other molecules), and other types of new delivery systems.

GenCefe Biotech provides comprehensive RNA modification, labeling, and conjugation services to meet the needs of different types of nucleic acid drug delivery. Backed by expertise and innovation, we're your partners in advancing research.

RNA Modifications:

Modification Type	Modifications	Modification Type	Modifications
Point Modification	2'OME	Special Modifications	Cholesterol
	dspacer		GalNAc
	dI		DBCO
	rI		Desthiobiotin
	dU		Cy5.5
	2'F		Cy7
	2'MOE		Hexynyl
	LNA		BHQ1
	m6A		BHQ2
	m5C		BHQ3
	m5U		MGB
End Modifications	Thiol		ATTO Series
	Amino Linker	Chimeric RNA	DNA\RNA
	Biotin	Chimeric RNA	RNA\2'F\2'OME\DNA
	Phosphorylation	Dual-Labeling	FAM-BHQ1
	spacer C9		FAM-TAMRA
	spacer C18		FAM-Dab
Fluorescent Labeling	FAM		TAMRA-BHQ2
	HEX		CY3-BHQ2
	Cy3		CY5.5-BHQ3
	Cy5		CY7-BHQ3
	TET
	VIC
	TAMRA

Modifications for Small Nucleic Acid Drug

Nucleic Acid Type	Chemical Modification and Fluorescent Labeling Services
siRNA	Sugar Modification: 2'-Ome , 2'-F, etc. Backbone Modification: PS (phosphorothioate) modification, etc. E nd modification: Chol (cholesterol), Biotin, Thiol, NH ₂ , etc. Fluorescent labeling: Cy3, Cy5, etc. Customized chemical modification and fluorescent labeling proposal design
Antisense (ASO)	Sugar Modification: 2'-OMe, 2'-MOE, 2'-F, LNA, etc. Base Modification: methylated cytosine (5-Me-dC), etc. Backbone Modification: PS (phosphorothioate) modification, etc. E nd modification: Chol (cholesterol), etc.
Aptamer	Sugar Modification: 2'-OMe, 2'-NH2, 2'-F, LNA, etc. Base Modification: methylated cytosine (5-Me-dC), etc. Backbone Modification: PS (phosphorothioate) modification, etc. E nd modification: 3'-inverted thymidine, Chol (cholesterol), Biotin, Thiol, NH ₂ , fluorescent dyes, etc.
Others	Custom chemical modification and fluorescent labeling services

Delivery System Customization and Conjugation Services

Delivery System	Features	Services
GalNAc (N-acetylgalactosamine)	GalNAc is the targeting ligand of the sialic acid receptor (ASGPR), which is mainly expressed on the surface of hepatic parenchymal cells. The conjugate of nucleic acid and GalNAc enters the cell through endocytosis to function.	Custom GalNAc Synthesis GalNAc-siRNA Synthesis GalNAc-ASO Synthesis GalNAc-miRNA Synthesis Customized design and synthesis of GalNAc delivery proposal
Peptide	Peptides have better membrane permeability and strong resistance to degradation. Therefore, conjugating oligonucleotides with various biological functions to peptides can not only improve the biological activity of oligonucleotides but also enhance their cell permeability.	Peptide Synthesis (drug peptides, custom peptides, RGD cyclic peptides, etc.) Peptide-siRNA Conjugation Peptide-ASO Conjugation Peptide-DNA Conjugation RGD-siRNA Conjugation
Lipid	LNP not only prevents degradation of delivered oligonucleotides and prolongs circulation time, but also facilitates oligonucleotide uptake, enhances intracellular transport, and facilitates endosomal escape.	LNP-siRNA Conjugation LNP-ASO Conjugation
PEG (polyethylene glycol)	The half-life of PEG-modified ASO in the body is significantly prolonged, and PEG can reduce the excretion rate of oligonucleotides and promote the uptake of nucleic acid drugs by corresponding cells.	PEG-Aptamer Conjugation PEG-siRNA Conjugation PEG-ASO Conjugation
Others	/	Custom delivery proposal design and synthesis

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EMAIL	PHONE	ONLINE FORM
[email protected]	+1 408-828-0438	ONLINE QUOTE SUBMISSION

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siRNA Synthesis

miRNA Synthesis

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