Pre-Designed siRNA Sets
Ready-to-Order siRNA Sets for Targeted Gene-Silencing Research
Pre-Designed siRNA Sets provide ready-to-order siRNA options for target-gene silencing research. GenCefe’s experienced design team has developed more than 50,000 pre-designed siRNA sets for human, mouse, and rat genes, with multiple pre-designed siRNA sequences targeting each selected gene.
Each set includes three or four independently designed siRNA sequences targeting the same gene, with positive-control, negative-control, and FAM-NC siRNA options. Gene-silencing performance can vary depending on experimental conditions. For applicable experiments with transfection efficiency above 80%, GenCefe provides a package guarantee: if the intended silencing effect is not achieved under the applicable conditions, one replacement set will be redesigned and synthesized free of charge, subject to the specific terms of the selected siRNA set.
Key Features:
Extensive Design Experience: More than 50,000 pre-designed siRNA sets developed for human, mouse, and rat genes, with three or four independently designed siRNAs targeting each selected gene.
Complete Control Options: Positive-control, negative-control, and FAM-NC siRNAs are available with the corresponding pre-designed sets, with order fulfillment starting from 4 days.
ISO-Certified Quality Management: siRNA production is supported by ISO 9001 and ISO 13485 certified quality management systems, with QC performed according to project specifications.
Gene-Silencing Guarantee: At least one siRNA in the set is guaranteed to achieve more than 70% silencing when transfection efficiency is above 80%, subject to the applicable terms.
Case Studies:
Case Study 1: Knockdown efficiency detection at mRNA level
 | Fig 1. Knockdown efficiency of siRNA Set detected by RT-qPCR Small interfering RNA (siRNA) successfully knocked down AC023644.1 in the human ovarian cancer epithelial cell lines A2780 and SKOV3 cells by RT-qPCR detection. Ref: Chen R et al., Heliyon, 10(15), e35004. |
Case Study 2: Knockdown efficiency detection at mRNA and protein level
 |  | Fig2. Knockdown efficiency of siRNA Set detected by RT-qPCR (left) and Western Blot (right) Three human SOX11-specific small interfering RNAs (siSOX11) or scrambled small RNAs (siNC) were transfected into periodontal ligament stem cells (PDLSCs), and qRT-PCR and Western blot verified that siSOX11 could effectively silence the mRNA and protein levels of SOX11. |
3 individual siRNA: 6 nmol/siRNA
Positive Control, Negative Control, FAM-NC: 3 nmol/each
4 individual siRNA: 12 nmol/siRNA
Positive Control, Negative Control, FAM-NC: 3 nmol/each
When cell transfection efficiency is greater than 80%, GenCefe guarantees that at least one siRNA pair in the selected set can achieve a gene-silencing effect of more than 70%. If this condition is not met, two new siRNA pairs will be redesigned and re-synthesized free of charge following comprehensive analysis.
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RNA Synthesis Services
Explore GenCefe’s RNA synthesis solutions, including custom RNA synthesis and modifications, siRNA, pre-designed siRNA sets, miRNA mimics and inhibitors, gRNA, circRNA, and more.
