Login
Register
Contact us
KR
GenCefe Biotech has established a professional oligonucleotide biological evaluation platform, providing a variety of customized services such as cell culture, screening, cell-based assay, protein detection, animal experiments, etc., providing one-stop services for nucleic acid drug researchers.
Cell Assay Platform:
early-stage drug development, in vitro mRNA & Protein efficacy, cytotoxicity,
early-stage off-target testing, primary cell testing, etc.
HT Molecular Detection Platform:
subcloning, off-target mimic construction, high-throughput mRNA regulation
detection, stability, and PK testing.
Protein Detection Platform:
protein level verification after oligonucleotide regulation of mRNA, detection
of nucleic acid & protein interaction, and detection of oligonucleotide
stability.
Primary Cell Platform:
the rapid test of delivery efficiency & activity of the delivery system
and oligonucleotide conjugate, early-stage off-target testing, PK testing.
Animal Platform:
early-stage PD, PK, TOX testing, rapid HDI modeling and evaluation of
oligonucleotides, and multiple drug delivery methods for target organs,
such as liver, lung, and CNS.
Service Specifications
| Services | Description |
| siRNA Screening |
Cell model construction
Primary cell culture and drug induction Cell transfection and optimization Customized detection methods development (gene level and protein level) Gene level: qPCR, vector construction, reporter gene detection, etc. Protein level: Western Blot, etc. |
| Binding assay of siRNA-GalNAc targeting hepatocytes |
GalNac-ASGPR transport assay
free-uptake test Primary cell isolation and detection GalNAc-siRNA long-acting study in vivo |
| Detection and Evaluation of siRNA Chemical Modifications |
Stability study
On-target activity studies (in vitro, in vivo) Off-target activity studies (in vitro, in vivo) |
| Early-stage in vivo and in vitro off-target assays |
Database search for potential off-target mRNAs and proteins
Luciferase reporter plasmid, RNAseq, rat toxicity, and other analysis and detection methods |
| Other | Customized oligonucleotide biological evaluation method development and experimental services |
Process Development & Technology Transfer
Synthesis process development, coupling process development, analytical method development and verification, quality control, and process scale-up.
IND (Investigational New Drug) Consultation and Technical Support
| REQUEST A QUOTE | ||
|---|---|---|
| PHONE | ONLINE FORM | |
| [email protected] | +1 408-828-0438 | ONLINE QUOTE SUBMISSION |
