Oligonucleotide biological evaluation platform
GenCefe Biotech has established a professional oligonucleotide biological evaluation platform, providing a variety of customized services such as cell culture, screening, cell-based assay, protein detection, animal experiments, etc., providing one-stop services for nucleic acid drug researchers.
Cell Assay Platform: early-stage drug development, in vitro mRNA & Protein efficacy, cytotoxicity, early-stage off-target testing, primary cell testing, etc.
HT Molecular Detection Platform: subcloning, off-target mimic construction, high-throughput mRNA regulation detection, stability, and PK testing.
Protein Detection Platform: protein level verification after oligonucleotide regulation of mRNA, detection of nucleic acid & protein interaction, and detection of oligonucleotide stability.
Primary Cell Platform: the rapid test of delivery efficiency & activity of the delivery system and oligonucleotide conjugate, early-stage off-target testing, PK testing.
Animal Platform: early-stage PD, PK, TOX testing, rapid HDI modeling and evaluation of oligonucleotides, and multiple drug delivery methods for target organs, such as liver, lung, and CNS.
Service Specifications
| Services | Description |
| siRNA Screening | Cell model construction Primary cell culture and drug induction Cell transfection and optimization Customized detection methods development (gene level and protein level) Gene level: qPCR, vector construction, reporter gene detection, etc. Protein level: Western Blot, etc. |
| Binding assay of siRNA-GalNAc targeting hepatocytes | GalNac-ASGPR transport assay free-uptake test Primary cell isolation and detection GalNAc-siRNA long-acting study in vivo |
| Detection and Evaluation of siRNA Chemical Modifications | Stability study On-target activity studies (in vitro, in vivo) Off-target activity studies (in vitro, in vivo) |
| Early-stage in vivo and in vitro off-target assays | Database search for potential off-target mRNAs and proteins Luciferase reporter plasmid, RNAseq, rat toxicity, and other analysis and detection methods |
| Other | Customized oligonucleotide biological evaluation method development and experimental services |
Process Development & Technology Transfer
Synthesis process development, coupling process development, analytical method development and verification, quality control, and process scale-up.
IND (Investigational New Drug) Consultation and Technical Support
